The role of quercetin in primary culture of ovine spermatogonial stem cells

The aim of the present study was to examine effect quercetin on survival and primary culture ovine spermatogonial stem cells (SSCs). two-time enzymatic digestion process employed obtain SSCs from lamb testes. In next step, use filtration differential plating methods caused an increase in number cell suspension resulting mechanical digestions. Mitomycin-C-treated Sertoli were used prepare feeder layer. then cultured identification colonies done through alkaline phosphatase staining specific gene expression ram’s (nanog Plzf). results methylthiazolyldiphenyl-tetrazolium bromide assay 72 h after with different treatments demonstrated that highest percentage for 5 μM 10 concentrations, respectively; however, compared control, no significant difference observed. comparison concentration equal greater than 20 a decrease (P < 0.05). Seven days culture, 40 substantial reduction mean colonies, control significantly reduced Plzf expression. Furthermore, higher decreased bcl-2 under Based findings study, is not recommended. It suggested function this antioxidant should be investigated differentiation SSCs.